Ameliorative effects of Senna Siamea Leaf Decoction in Loperamide-Induced Constipated Albino Rats.

Abstract

This study sought to assess the ameliorative effects of Senna siamea leaf decoction in loperamide-induced constipated rats. Completely Randomized Design was employed for this study, and a total of 20 Albino rats were randomly assigned into 4 treatment groups after two weeks of acclimatization. With each treatment having 5 replicates. Treatment 1 (Control) received normal saline throughout the experimental period. Treatment 2 was administered with a dosage of 0.75 mg of loperamide in 5ml of water (5 mg/kg body weight) for 4 days. Treatment 3 received normal saline for 4 days and then 5 ml of Senna siamea leaf decoction with a dose concentration of 33.33 g/kg body weight for a duration of 7 days. Treatment 4 was administered with a dosage 0.75 mg of loperamide in 5 ml of water for 4 days, followed by a dosage of 5 ml of Senna siamea leaf decoction for a duration of 7 days by oral gavage. The study evaluated feed and water intake, faecal pellets and faecal moisture, kidney and liver functions, and kidney and liver histology. There was no significant difference in feed and water intake during the period of constipation (p>0.05). There was variability in feed intake on the fourth day of the Senna siamea leaf decoction administration. Water intake increased during the period of the Senna siamea leaf decoction administration. Treatment 2 recorded high water intake on the seventh day of the Senna siamea leaf decoction administration (p<0.01). Treatment 2 recorded fewer faecal pellets compared to the control group throughout the experimental period (p<0.01). Treatments 3 and 4 recorded more faecal pellets (p<0.01). Faecal moisture reduced in treatment 2 (p<0.01). The data on kidney function showed that treatment 2, treatment 3 and treatment 4 recorded decreased urea levels (p<0.05) compared to the control. Cl- level was lower (p<0.05) in treatment 2 compared to the control. No differences were observed in the liver function parameters among the treatment groups throughout the experimental period (p>0.05). Kidney and liver tissues showed normal architecture at the end of the experiment. The research findings suggest that Senna siamea leaf decoction has laxative effect on loperamide-induced constipation in Albino rats.