Effects of Aqueous Garlic Extract on the Reproductive Function and Hematology of Male Wistar Rats treated with Monosodium Glutamate.

Abstract

This study aimed to evaluate the effects of different doses of aqueous garlic extract on the reproductive function and hematology of male Wistar rats treated with monosodium glutamate. A total of 25 male Wistar rats, with an average body weight of 135 g, were randomly divided into 5 treatment groups, with 5 replicates per group. Treatment 1 (control) was administered normal saline. Treatment 2 was given 120 mg/kg BW of MSG. Treatment 3 was administered 120 mg/kg BW of MSG and 500 mg/kg BW of AGE. Treatment 4 was administered 120 mg/kg BW of MSG and 750 mg/kg BW of AGE. Treatment 5 was administered 120 mg/kg BW of MSG and 1000 mg/kg BW of AGE by oral gavage for 21 days. The study assessed the sperm count, motility, and morphology of epididymal sperm, white blood cells (WBC), granulocytes (GRA), red blood cells (RBC), haematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), hemoglobin (HGB), lymphocytes (LYM), platelets (PLT), platelet large cell ratio (P-LCR) counts, reproductive organ’s weight, and testis histomorphometry at the end of the experiment. There was no significant difference in sperm count among the treatments (p > 0.05). Sperm motility increased in treatments 4 and 5 (p < 0.05). Immotile sperm were high in treatment 2. Treatments 3, 4, and 5 had a high level of normal sperm morphology. The weights of both the left and right testes were higher (p < 0.05) in treatment 5. Treatment 2 had decreased levels of WBC, RBC, and P-LCR in comparison to the control group. However, treatment 2 recorded increased levels of GRA. There were deformities in the seminiferous tubules of treatment 2, but they were considerable in treatments 3 and 4. Treatment 5 saw a reduction in the size of the seminiferous tubules. The research findings suggest that garlic has a dose dependent impact on mitigating the negative effects of MSG on the reproductive function and hematology of male Wister rats.